Abstract: In substation operation and maintenance management, the heavy inspection task and bad environment make it difficult to guarantee the quality of manual inspection. Therefore, the safe and stable operation of power grid is seriously affected. In order to effectively improve the reliability, security, economy and efficiency of power grid operation, and fundamentally realize the unattended operation and maintenance management goal of substation. It is very necessary to install intelligent inspection robot in substation for auxiliary inspection. The intelligent inspection robot has the functions of inspection, infrared temperature measurement, meter identification, etc. It has its own unique advantages in substation operation and maintenance management. Taking a substation as an example, this paper explores the application of intelligent inspection robot in substation operation and maintenance management in single station mode and centralized control mode. Through the application effect analysis, the intelligent inspection robot can ensure the quality of substation operation and maintenance, which is worthy of further promotion and application.